Flow Cytometry Core Laboratory

[Electron Microscopy] [Flow Cytometry] [Histology] [Imagecore] [Nucleotide Sequencing]
[Peptide Core] [Transgenic Core] [VTPB Central Stores] [VTPB Grading Facility]

The facility operator is Dr. Roger Smith III (rosmith@cvm.tamu.edu), 1-979-845-5167. For more information, or to arrange assays, contact Dr. Smith.

Instrumentation:
- Becton-Dickinson FACSCalibur™
  - argon laser (488nm)
  - 3-color fluorescence
- Becton-Dickinson FACSVantage SE™
  - argon (488nm) and HeNe (633nm) lasers; solid-state UV (355nm) laser to be added
  - up to 6-color fluorescence
  - with TurboSort option

FACSCalibur in room SAH2020

FACSVantage in room SAH2020

Location:

The Flow Cytometry facility is located in room 2020, Small Animal Hospital (SAH) Building.

From the Main Entrance to the College of Veterinary Medicine, walk past the vending machines, then turn right for a few feet. Turn left at the first hallway and walk up the wide staircase (marked "Stairs to CVM Foyer" on the map below). The Clinical Pathology Lab is the 3rd door to your left.

From the Veterinary Medical Research Bldg (to the left of the map), you can use the Small Animal Hospital nearest to the VMR. Take the stairs (labeled "S" on the map) and follow the hall (orange on the map) to the Clinical Pathology Lab.

The door to the Clinical Pathology door is always open.
Please check in with the Front Desk personnel.

Map to FCM Lab

Software:
- CellQuest™ (Becton-Dickinson)
- FlowJo™ (TreeStar)
- ModFit LT™ (Verity Software House)

Capabilities:
- Detection of the following fluorochromes:
  - Both FACSCalibur & FACSVantage SE:
    - Fluorescein, CFSE, Alexa Fluor 488
    - GFP
    - R-phycoerythrin
    - R-phycoerythrin-cyanine 5 (PE-Cy5) tandem dye
    - Peridinin chlorophyll protein (PerCP)
    - Propidium iodide
    - 7-aminoactinomycin D
    - Doxorubicin (adriamycin)
    - Rhodamine 123
    - and others (please check)
  - FACSVantage SE only:
    - Allophycocyanin (APC)
    - Cyanine 5
    - Alexa Fluor 647
    - Alexa Fluor 700
    - future: Hoechst dyes (33528, 33342)
    - future: 4',6-diamidino-2-phenylindole (DAPI)
    - future: Alexa Fluor 350
    - and others (please check)
  - Cell Sorting

Assays Supported:
- Immunofluorescence (surface and intracellular antigens, quantitative)
- DNA and cell cycle content analysis (using ModFit LT software)
- Apoptosis (annexin V binding, caspase activation, TUNEL)
- Cell Proliferation (CFSE)
- Phagocytosis of fluorescent beads
- Functional expression of p-glycoprotein (using doxorubicin)

User Fees:
- Data Acquisition: $50 per hour ($15 minimum fee)
- Cell Sorting: $75 per hour (plus $100 set up fee)