Automated DNA Sequencing

[DNA Core Facility] [Fluorescent Genotyping]

All sequencing is done with the ABI 3130 Genetic Analyzer, an automated high-through put capillary electrophoresis system used for analyzing fluorescently labeled DNA fragments. This machine injects sixteen samples at one time into a capillary array 80cm long. During electrophoresis a laser excites the labeled DNA and the resulting fluorescence is collected by a CCD camera. The 3130 Data Collection Software reads and interprets the data which is then displayed an as electropherogram.

Sequencing Results

Pricing:

Sequence Reaction	$15.00	Requester provides template and primer; we perform the sequence reaction
Ready-to-run Reaction	$3.00	Requester provides precipitated sequence
Big Dye Version 2.0	$80.00	80 µL (10 reactions) of fluorescent label
Spin 50 mini columns	$16.00	10 columns to purify and clean sequence reaction

	Contact Technician: Amy Boedeker 979-862-3991 derrlab@cvm.tamu.edu
	Sequencing request form (HTTP:)- front / back Alternate method (FTP:)- Press Here
	Troubleshooting Notes for DNA sequencing
	Information on ABI PRISM sequencing kit

New Big Dye Kit:

The dGTP Kit was developed for use with difficult templates where the standard Big Dye kit gives early signal loss. GT rich, G rich and templates with certain sequencing contexts or motifs are best sequenced with the dGTP kit.

WEB LINKS:

Laboratory of Molecular Systematics and Evolution

Qiagen DNA sequencing and Genomic